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The Effect of Changing ISO on Depth of Field
We’re used to upping the ISO to speed up the shutter speed, but few people consider what it’s going to do the aperture. Increasing the ISO makes the camera more sensitive, so allows a faster shutter or a smaller aperture, which changes the depth of field.
1/8000th, f2.8, 400ISO
1/8000th, f5.6, 1600ISO
1/8000th, f13, 6400ISO
You can see that the droplets stay the same, but the background comes into focus as the ISO increases.